The Simultaneous Determination of Some Water-Soluble Vitamins in Gum of Acacia nilotica by High Performance Liquid Chromatography

A rapid, simple and precise method by HPLC (high performance liquid chromatography) has been developed for simultaneous determination of water-soluble vitamins as thiamine(B1), nicotinamide(B3), panthotenic(B5), pyridoxine(B6) and biotin(B8) in gum of Acacia nilotica using enzymatic hydrolysis. The method uses a C18 column (4.6×150 mm, 5µm). Mobile phase such as methanol 0.1M, sodium dihydrogen phosphate (pH = 2.5), (10:90 v/v) is found most suitable for rapid separation and identification of this water –soluble vitamins. Good linearity was observed between the concentration of analytes and peak area (r = 0.9999). Each vitamin was quantitatively determined at its maximum wavelength. Recovery percentages ranged from 97% to 99%.